Tissue preparation

NDT400a


Tissue cryoprotection & freezing:

Fixed tissue will be infiltrated with our unique tissue cryoprotection solution (cf. Products, Cat. #NDT302) and then rapidly frozen in isopentane precooled to -70°C. The frozen tissue will be stored at -75°C before sectioning.

Remarks:

  • A quotation is required before placing an order.
  • The investigator needs to provide fixed tissue.

Embedding tissue in paraffin:

Following a thorough wash in distilled water, fixed tissue will be dehydrated through graded ethanol, cleared in xylenes, and then impregnated and embedded in paraffin.

Remarks:

  • A quotation is required before placing an order.
  • The investigator needs to provide fixed tissue.
  • We use various types of paraffin with the melting point ranging from 50-56°C. The investigator may choose the type of paraffin for a particular purpose.

Embedding Tissue in Epoxy Resin:

Please contact us for more information.

Terms and Conditions

  • For quality assurance of our service, it is recommended that you discuss with us for preferred perfusion protocol and histology and/or immunolabeling protocols.
  • It is suggested that you use Gel-coated microscopic slides for tissue mounting and 0.17um-thick coverslips.
  • A 15% of the fee will be due upon authorization of the study; and the remaining fee will be due upon delivery of study results.
  • Progress of the service is contingent upon staining quality of tissues, operated by the independent contractor.
  • Should early termination occur, Neurodigitech will prorate the cost incurred and invoice the Sponsor. The first portion of the fee is non-refundable.