GolgiChrome staining Service

NDT503

GolgiChromeTM staining Service (Double-stainiing of Golgi impregnation and immunohistochemistry)

This invention is designed to achieve the simultaneous visualization of Golgi-Cox and immunofluorescence of individual neurons using Confocal Laser Scanning Microscopy (CLSM).

The main advantages of this method are as follows:

  • Highest visualization of structural details along with the neurochemical nature of the neuron;
  • Characterization of antigens with specific antibodies
  • Anatomical interactions among neuronal elements;
  • The possibility of using confocal laser scanning microscope (CLSM) and accordingly 3D reconstructions and modeling;
  • Savings of laboratory animals, when up to now it was necessary to perform the two techniques separately and thus on different animals.
  • Consequent substantial advantages in terms of time and cost saving, together with a higher quality than traditional techniques.

The following examples (Spiga et al., 2012) demonstrate the co-localizations of Golgi-cox impregnated neurons immunoreactive to Tyrosine Hydroxylase (TH) (Figure 1), TH and Postsynaptic Density 95 (PSD-95) (Figure 2), TH, PSD-95 or Synapsin (SynI) (Figure 3), and TH, PSD-95 or SynI of a fully impregnated pyramidal cell (Figure 4).

Figure 1. Golgi + Tyrosine hydroxylase (TH)​ immunohistochemistry in the striatum.

Figure 2. Golgi + TH + PSD95 immunohistochemistry in the substantia nigra (SN, upper panel) and anterior commissure (ACA, lower panel).

Figure 3. Golgi + TH + PSD95 or Synapsin (SynI) immunohistochemistry in the dendritic segments of the medial spiny neurons (MSN) of the striatum.

Figure 4. Golgi + TH + PSD95 or SynI immunohistochemistry of the prefrontal prelimbic zone​ of the rat brain.

*Analysis of Variance (ANOVA) test will be performed!

Terms and Conditions

  • For quality assurance of our service, it is recommended that you discuss with us for preferred perfusion protocol and histology and/or immunolabeling protocols.
  • It is suggested that you use Gel-coated microscopic slides for tissue mounting and 0.17um-thick coverslips.
  • A 15% of the fee will be due upon authorization of the study; and the remaining fee will be due upon delivery of study results.
  • Progress of the service is contingent upon staining quality of tissues, operated by the independent contractor.
  • Should early termination occur, Neurodigitech will prorate the cost incurred and invoice the Sponsor. The first portion of the fee is non-refundable.