Zeiss Axioplan 2 upright digital microscope with an extended travel stage that holds 8-slides per run, controlled by LUDL MAC5000 is used to acquire images of
Region of Interest (ROI) using Zeiss AxioCam
and/or MacroFireŽ Digital Microscope CCD Camera (2048 x 2048) (Lens type: 2.5x, 5x, 10x, 20x, 40x, 63x
oil, and 100x oil immersion). Final image files can be saved in a variety of
formats including TIFF, PICT, BMP,
GIF, etc.
With
stabilized stage control and aligned fiducial during acquisition process, the stack of
2D images will be generated and made ready for subsequent 3D reconstruction of ROIs
(optional). Note: The complete series of aligned 2D image
stack will be suitable for morphometric analysis (NDT501 service, see
below).
Zeiss digital microscopic imaging platform (AxioPlan 2 digital
microscopy)
3D reconstructed hippocampus of a mouse brain
!
Animated 2D image series of a mouse brain, acquired by Zeiss Axioplan 2 digital microscopy
Terms and Conditions
For quality assurance
of our service, it is recommended that you discuss with us for preferred
perfusion protocol and histology and/or immunolabeling protocols.
It is suggested that
you use Gel-coated microscopic slides for tissue mounting and
0.17um-thick coverslips.
A 15% of the fee will
be due upon authorization of the study; and the remaining fee will be due upon
delivery of study results.
Progress of the service
is contingent upon staining quality of tissues, operated by the independent
contractor.
Should early
termination occur, Neurodigitech will prorate the cost incurred and invoice the
Sponsor. The first portion of the fee is non-refundable.
NDT500-B: Digital scanning of histology slides
The alternative image acquisition method, PathScan Enabler scanner is available to
allow for high-speed scanning of an entire whole mounted histology (1 x 3 inch cover
slipped glass slide). Using a low-power objective is efficient to acquire large field of view of ROIs, rendering made-ready image stacks for post image process, including stitching and tiling then morphological analysis (optional). The scanner combined with
in-house software programs is capable of scanning an area of 1.42 x 0.85 inches
(36.14 x 21.59 mm), resulting in useable images up to 10,248 x 6,120 pixels,
uninterpolated. Note: File size can be as large as 188 megabytes. Final image
files can be saved in a variety of formats including TIFF, PICT, BMP, GIF, etc.
This is a low-cost but effective approach for acquiring representative high-resolution images of
histology slides.For digital
acquisition of serial section images,
it is recommended to use NDT500-A service due to its stable microscopic stage control and alignment functions, tilting and stitching of serial images of ROIs.
PathScan Enabler IV (courtesy by Meyer Instruments)
A sagittal view of a mouse embryonic brain (courtesy by Meyer Instruments)
