| The £]-galactosidase
gene is often used
as a reporter gene,
¡§marker¡¨ that is
widely used for
analysis of mutationally
altered genes as
well as gene regulation.
The expression of
£]-galactosidase
gene can be detected
by histochemical
staining of tissue
sections or cells.
The method is based
upon the ability
of £]-galactosidase
to hydrolyze 5-bromo-4-chloro-3-indolyl-£]-D-galactopyranoside
(X-Gal), resulting
in a blue staining
in the cells expressing
£]-galactosidase1.
This service
includes tissue
preparation, sectioning,
histochemical
staining, counterstaining
(optional), and
coverslipping.
As a result, your
will receive X-Gal
stained sections
ready for microscopic
observations.
Remarks:
¡P A quotation
is required before
placing an order.
¡P The investigator
needs to provide
unfixed and freshly
frozen tissue.
¡P Please contact
us for more information.
Reference:
1. Horvitz, J
P et al. (1964)
J. Med. Chem.
7:574.
|
