|The ß-galactosidase gene is often used as a reporter gene, ¡§marker¡¨ that is widely used for analysis of mutationally altered genes as well as gene regulation. The expression of ß-galactosidase gene can be detected by histochemical staining of tissue sections or cells. The method is based upon the ability of ß-galactosidase to hydrolyze 5-bromo-4-chloro3-indolyl-ß-D-galactopyranoside (X-Gal), resulting in a blue staining in the cells expressing ß-galactosidase1 (See example)
This service includes tissue preparation, sectioning, histochemical staining, counterstaining (optional) and coverslipping. As a result, you will receive X-Gal stained sections ready for microscopic observations.
1. A quotation is required before placing an order.
2. The investigator needs to provide tissue fixed with special fixative.
3. Please contact us for more information.
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1. Horvitz, J P et al. (1964) J. Med. Chem. 7:574.