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NDT403-e
NDT403-e X-Gal histochemistry
The ß-galactosidase gene is often used as a reporter gene, ¡§marker¡¨ that is widely used for analysis of mutationally altered genes as well as gene regulation. The expression of ß-galactosidase gene can be detected by histochemical staining of tissue sections or cells. The method is based upon the ability of ß-galactosidase to hydrolyze 5-bromo-4-chloro3-indolyl-ß-D-galactopyranoside (X-Gal), resulting in a blue staining in the cells expressing ß-galactosidase1 (See example)

This service includes tissue preparation, sectioning, histochemical staining, counterstaining (optional) and coverslipping. As a result, you will receive X-Gal stained sections ready for microscopic observations.

Remarks:
1. A quotation is required before placing an order.
2. The investigator needs to provide tissue fixed with special fixative.
3. Please contact us for more information.

Please contact us for more information.

Reference:
1. Horvitz, J P et al. (1964) J. Med. Chem. 7:574.

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