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NDT403-b
NDT403-b Tissue preparation & Golgi-Cox Staining with FD Rapid GolgiStain
Golgi-Cox impregnation1,2 has been one of the most effective techniques for studying both the normal and abnormal morphology of neurons as well as glia. Using Golgi techniques,subtle morphological alterations in neuronal dendrites and dendritic spines have been discovered in the brains of animals treated with drugs as well as in the postmortem brains of patients with neurological diseases3,4. However, the complex and time-consuming process of Golgi staining has been a major obstacle to the widespread application of this technique.

FD Rapid GolgiStainTM Kit (NDT104), designed based on the principle of the methods described by Ramon-Moliner2, Glaser and Van der Loos5, has overcome most problems associated with the Golgi-Cox technique. FD Rapid GolgiStain Kit has been tested extensively in the brains from several species of animals as well as in specimens of the postmortem human brain. This kit has not only significantly simplified and improved the Golgi-Cox technique but also proven to be extremely sensitive and reliable for demonstrating morphological details of neurons and glia, especially dendritic spines.

This service includes tissue preparation, sectioning, staining, coverslipping and slide labeling. As a result, you will receive up to 40 Golgi-Cox-impregnated sections ready for microscopic observations per brain or per tissue block.

Procedure: Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C . The frozen tissue will be cut on a cryostat and mounted on gelatin-coated microscope slides. Subsequently, sections cut through various levels (or the levels of your choice) will be processed with NDT104-FD Rapid GolgiStain kit.  Also, please see more Golgi impregnated samples (click) here!


Remarks:
1. A quotation is required before placing an order.
2. The investigator needs to provide tissue fixed with special fixative provided by NeuroDigiTech.
3. Please contact us for more information.

Reference:

1. Corsi P: Camillo Golgi'¦s morphological approach to neuroanatomy. In Masland RL, Portera-Sanchez A and Toffano G (eds.), Neuroplasticity: a new therapeutic tool in the CNS pathology, pp 1-7. Berlin: Springer, 1987.

2. Ramon-Moliner E: The Golgi-Cox technique. In Nauta WJH and Ebbesson SOE (eds.), Contemporary Methods in Neuroanatomy. pp 32-55, New York: Springer, 1970.

3. Graveland GA, Williams RS and DiFiglia M: Evidence for degenerative and regenerative changes in neostriatal spiny neurons in Huntington¡¦s disease. Science 227:770-773, 1985.

4. Robinson TE and Kolb B: Persistent structural modification in nucleus accumbens and prefrontal cortex neurons produced by previous experience with amphetamine. J. Neurosci. 17:8491-8497, 1997.

5. Glaser ME and Van der Loos H: Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain. J. Neurosci. Methods 4:117-125, 1981.

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