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This service
includes tissue
preparation, sectioning,
silver-staining,
mounting, coverslipping
and labeling of
slides. As a result,
you will receive
up to 40 silver-stained
sections per brain
or per tissue
block ready for
microscopic observations.
To express our
appreciation,
a set of Nissl
(or H & E)
stained sections
adjacent to those
used for silver-staining
will also be provided
for you without
additional cost.
Procedure:
Following cryoprotection,
tissue will be
rapidly frozen
in isopentane
pre-cooled to
-70¢XC. The frozen
tissue will then
be cut on a cryostat
and collected
in our unique
section cryoprotection
solution (cf.
Product
NDT301). Subsequently,
sections cut through
various levels
(or the levels
of your choice)
will be processed
free-floating
for the detection
of neurons undergoing
degeneration with
Gallyas silver
staining technique1.
This technique
was originally
described by Gallyas
et al.1 and later
modified2. It
is particularly
useful for the
detection of early
neuronal injury
in the central
nervous system
of experimental
animals. Typically,
both the neuronal
perikarya and
processes are
silver-stained.
This technique
permits the morphological
categorization
of damaged neurons
and the detection
of subtle changes
in the morphology
of cell bodies
and processes
(cf. photo samples
below).
Remarks:
¡P A quotation
is required before
placing an order.
¡P The investigator
needs to provide
tissue fixed by
perfusion with
a special fixative.
¡P Please contact
us for more information.
References:
1. Gallyas et
al. (1990) Acta
Neuropath. 79,
620-628.
2. Du et al. (1998)
Neuroscience 82,
1165-1178.
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