This service includes tissue preparation, sectioning, silver-staining, mounting, coverslipping and labeling of slides. As a result, you will receive up to 40 silver-stained sections per brain or per tissue block ready for microscopic observations. To express our appreciation, a set of Nissl (or H & E) stained sections adjacent to those used for silver-staining will also be provided for you without additional cost.
Procedure: Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70ºC. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Product NDT301). Subsequently, sections cut through various levels (or the levels of your choice) will be processed free-floating for the detection of neurons undergoing degeneration with Gallyas silver staining technique1.
This technique was originally described by Gallyas et al.1 and later modified2. It is particularly useful for the detection of early neuronal injury in the central nervous system of experimental animals. Typically, both the neuronal perikarya and processes are silver-stained. This technique permits the morphological categorization of damaged neurons and the detection of subtle changes in the morphology of cell bodies and processes (cf. photo samples below).
1. A quotation is required before placing an order.
2. The investigator needs to provide unfixed frozen tissue.
3. Please contact us for more information.
1. Gallyas et al. (1990) Acta Neuropath. 79, 620-628.
2. Du et al. (1998) Neuroscience 82, 1165-1178.