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NDT401-ca Tissue preparation & immunogold labeling with 1 primary antibody on free-floating sections

This service includes tissue preparation, sectioning, immunolabeling, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunolabeled sections per brain or per tissue block ready for microscopic observations. To express our appreciation, a set of Nissl (or H & E) stained sections adjacent to those used for immunostaining will also be provided for you without additional cost.

Procedure: Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70ºC. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Product NDT301). Subsequently, the sections cut from various levels (or the levels of your choice) will be processed free-floating for immunostaining with 1 specific antibody according to immunogold labeling technique1 (cf photo samples below).

Tyrosine hydroxylase-immunoreactivity in the rat substantia nigra. 30 £gm cryostat section cut coronally from the midbrain of a normal rat. This section was processed free-floating according to the immunogold labeling technique. Note densely labeled neurons in the compact area of the substantia nigra.

Tyrosine hydroxylase-immunoreactivity in the rat substantia nigra. The high magnification of the substantia nigra as shown above. Note that dense black deposits in the cytoplasm of neurons are actually metallic silver grains, which can also be viewed under a microscope with a darkfield condenser.

1. A quotation is required before placing an order.
2. The investigator needs to provide fixed tissue and the specific antibody.
3. Please contact us for more information.

Humbel B. M., Sibon O. C. M., Stierhof Y.-D. and Schwarz H. (1995) Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and In Situ Hybridization experiments. J. Histochem. Cytochem. 43, 735-737.