|This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations. To express our appreciation, a set of Nissl (or H & E) stained sections adjacent to those used for immunostaining will also be provided for you without additional cost.
Procedure: Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70ºC. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated microscope slides (see product). Subsequently, sections cut from various levels (or the levels of your choice) will be processed on slides for immunostaining with one specific antibody according to the indirect immunofluorescence method1 (cf. photo samples below).
Substance P-immunofluorescence in the spinal cord. 10-micron cryostat section was cut transversely from the chicken spinal cord. This section was processed on slide according to the indirect fluorescence method (for details, cf. J. Comp. Neurol. 278:253-264, 1988). Note substance P containing fibers mainly in the dorsolateral funiculus, Lissauer¦s tract and the dorsal horn.
Substance P-immunofluorescence in the spinal cord. An 10-micron cryostat section of the chicken spinal cord was processed as described above. Note dense substance P immunoreactivity in the dorsolateral funiculus and the dorsal horn.
Vasoactive intestinal polypeptide-immunofluorescence in the spinal cord. An 10-micron cryostat section of the chicken spinal cord was processed on slide according to the indirect fluorescence method. Note 2 large neurons containing vasoactive intestinal polypeptide in the nucleus of the dorsolateral funiculus (for details, cf. J. Comp. Neurol. 278:253-264, 1988).
1. A quotation is required before placing an order.
2. The investigator needs to provide fixed tissue and the specific antibody.
3. Please contact us for more information.
1. Coons, A.H. (1958) Fluorescent antibody methods. In J.F. Danielli (ed): General Cytochemical Methods. New York: Academic Press, pp. 399-422.