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NDT401-ab Tissue preparation & colorimetric immunostaining with 1 primary antibody on sections mounted on slides
This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations. To express our appreciation, a set of Nissl (or H & E) stained sections adjacent to those used for immunostaining will also be provided for you without additional cost.

Procedure: Following cryopretection, tissue will be rapidly frozen in isopentane pre-cooled to -70ºC. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated slides (see detail). Sections cut from various levels (or the levels of your choice) will be processed on slides for immunostaining with 1 specific antibody according to the avidin-biotin-complex (ABC) method1( cf. photo samples below).

Cytokeratin 18-immunostained section counterstained with cresyl violet. This 12 £gm frozen section of the rat prostate was processed for Cytokeratin 18-immunoreactivity (brown) and was then counterstained with cresyl violet solution (cf. Product NDT202) (click to see enlarged photo).

CD31-immunostained section counterstained with hematoxylin. This 7 £gm paraffin section of the mouse ear was processed for CD31-immunoreactivity (brown) and was then counterstained with hematoxylin solution (cf. Product NDT204) (click to see enlarged photo).

1. A quotation is required before placing an order.
2. The investigator needs to provide fixed tissue and the specific antibody.
3. Please contact us for more information.

1. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.

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