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NDT401-ab
NDT401-ab Tissue preparation & colorimetric immunostaining with 1 primary antibody on sections mounted on slides
This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations. To express our appreciation, a set of Nissl (or H & E) stained sections adjacent to those used for immunostaining will also be provided for you without additional cost.

Procedure: Following cryopretection, tissue will be rapidly frozen in isopentane pre-cooled to -70ºC. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated slides (see detail). Sections cut from various levels (or the levels of your choice) will be processed on slides for immunostaining with 1 specific antibody according to the avidin-biotin-complex (ABC) method1( cf. photo samples below).



Cytokeratin 18-immunostained section counterstained with cresyl violet. This 12 £gm frozen section of the rat prostate was processed for Cytokeratin 18-immunoreactivity (brown) and was then counterstained with cresyl violet solution (cf. Product NDT202) (click to see enlarged photo).

CD31-immunostained section counterstained with hematoxylin. This 7 £gm paraffin section of the mouse ear was processed for CD31-immunoreactivity (brown) and was then counterstained with hematoxylin solution (cf. Product NDT204) (click to see enlarged photo).

Remarks:
1. A quotation is required before placing an order.
2. The investigator needs to provide fixed tissue and the specific antibody.
3. Please contact us for more information.

Reference:
1. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.

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