|Counterstain is often used to reveal cells that are not labeled by the primary staining. For example, tissue sections that have been previously processed for immunohistochemistry or other labelings (e.g. TUNEL, ISNT) may be counterstained with histological dyes such as thionin (blue), neutral red (red), or methyl green (green) (cf. photo samples below).
NDT105-a Counterstain with cresyl violet (with NDT202)
NDT105-b Counterstain with eosin Y (with NDT203)
NDT105-c Counterstain with hematoxylin (with NDT204)
NDT105-d Counterstain with methyl green (with NDT205)
NDT105-e Counterstain with neutral red (with NDT206)
NDT105-f Counterstain with thionin (with NDT201)
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Parvalbumin-immunostained section counterstained with thionin. 30-micron cryostat section through the medial entorhinal cortex of a rat that survived for 24 hr after kainic acid administration, showing the preferential loss of neurons in layer III and relative resistance of parvalbumin neurons (for details, cf. J. Neurosci. 15:6301-6313, 1995) (click to see enlarged photo).
TUNEL-labeled section counterstained with methyl green. This 10-micron paraffin section cut from a dorsal root ganglion of a mouse embryo (E17). The section was processed for detecting neuronal apoptosis (brown) with NDT102 NeuroApop Kit and then counterstained with NDT205 methyl green (click to see enlarged photo).
Cytokeratin 18-immunostained section counterstained with cresyl violet. This 12-micron frozen section of the rat prostate was processed for Cytokeratin 18-immunoreactivity (brown) and was then counterstained with NDT202 cresyl violet solution (click to see enlarged photo).
CD31-immunostained section counterstained with hematoxylin. This 7-micron paraffin section of the mouse ear was processed for CD31-immunoreactivity (brown) and was then counterstained with NDT204 hematoxylin solution (click to see enlarged photo).
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