| Counterstain
is often used
to reveal
cells that
are not labeled
by the primary
staining.
For example,
tissue sections
that have
been previously
processed
for immunohistochemistry
or other labelings
(e.g. TUNEL,
ISNT) may
be counterstained
with histological
dyes such
as thionin
(blue), neutral
red (red),
or methyl
green (green)
(cf. photo
samples below).
NDT105-a
Counterstain
with cresyl
violet (with NDT202)
NDT105-b
Counterstain
with eosin
Y (with NDT203)
NDT105-c
Counterstain
with hematoxylin
(with
NDT204)
NDT105-d
Counterstain
with methyl
green (with NDT205)
NDT105-e
Counterstain
with neutral
red (with NDT206)
NDT105-f
Counterstain
with thionin
(with NDT201)
(See
details
in "Products"
-- "Ready-to-Use
Staining
Solutions")
Parvalbumin-immunostained
section
counterstained
with thionin.
30 £gm cryostat
section
through
the medial
entorhinal
cortex of
a rat that
survived
for 24 hr
after kainic
acid administration,
showing
the preferential
loss of
neurons
in layer
III and
relative
resistance
of parvalbumin
neurons
(for details,
cf. J. Neurosci.
15:6301-6313,
1995) (click
to see enlarged
photo).
TUNEL-labeled
section
counterstained
with methyl
green. This
10 £gm paraffin
section
cut from
a dorsal
root ganglion
of a mouse
embryo (E17).
The section
was processed
for detecting
neuronal
apoptosis
(brown)
with NDT102
NeuroApop
Kit and
then counterstained
with NDT205
methyl green (click
to see enlarged
photo).
Cytokeratin
18-immunostained
section
counterstained
with cresyl
violet.
This 12
£gm frozen
section
of the rat
prostate
was processed
for Cytokeratin
18-immunoreactivity
(brown)
and was
then counterstained
with NDT202
cresyl violet
solution (click
to see enlarged
photo).
CD31-immunostained
section
counterstained
with hematoxylin.
This 7 £gm
paraffin
section
of the mouse
ear was
processed
for CD31-immunoreactivity
(brown)
and was
then counterstained
with NDT204
hematoxylin
solution (click
to see enlarged
photo).
Remark:
A quotation
is required
before placing
an order.
|
