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Nissl stain
NDT400-c Routine Staining
Nissl stain (thionin, cresyl violet, or Azure II)

Several basophilic dyes can be used for Nissl staining. Thionin and azure II impart a deep blue color to Nissl substance of neurons and a pale blue color to the nuclear chromatin of both neuronal and non-neuronal cells. A similar staining pattern is also obtained with cresyl violet, which yields a violet color (cf. photo samples below).

Nissl stain with thionin (cf. Product NDT201)
Nissl stain with cresyl violet (cf. Product NDT202)
Nissl stain with azure II (cf. Product NDT207)

Nissl stain. 20-micron cryostat section cut transversely from the rat spinal cord was stained with NDT202 crysel violet.

Nissl stain. High magnification of the ventral horn from the same section as shown above.

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