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NDT400-a Tissue Preparation

Tissue cryoprotection & freezing
Fixed tissue will be infiltrated with our unique tissue cryoprotection solution (see detail) and then be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will be stored at -75°C before sectioning.

Remarks:
A quotation is required before placing an order.
The investigator needs to provide fixed tissue.

Embedding tissue in paraffin
Following a thorough wash in distilled water, fixed tissue will be dehydrated through graded ethanol, cleared in xylenes, and then impregnated and embedded in paraffin. The embedded tissue will be stored at 4°C before sectioning.

Remarks:
1. A quotation is required before placing an order.
2. The investigator needs to provide fixed tissue.
3.
We use various types of paraffin with the melting point ranging from 50-56°C. The investigator may choose the type of paraffin for a particular purpose.

Embedding tissue in epoxy resin

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