Detection of neurodegeneration with NeuroSilver Kit (NDT103). A: Section (40-micron) through the dentate gyrus of the hippocampus from a rat injected with kainic acid (10 mg/kg, s.c.), showing degenerating neurons and processes (black) in the polymorphic layer (pl) and the molecular layer (ml), respectively. B: Coronal section (30-micron) through the septum of a rat, killed at 10 days following a unilateral transection of the fimbria. Note degenerating axons (indicated by the arrow) in the ipsilateral fornix. C: Horizontal section (40-micron) through the hippocampal dentate gyrus of a rat killed at 5 days after the intra-entorhinal injection with aminooxyacetic acid. Note numerous degenerating axon terminals (arrowheads) in the middle zone of the molecular layer (ml), the terminal field of entorhinal neurons killed by the drug injection (for details, cf. Neuroscience 82:1165, 1998). D: Coronal section (40-micron) through the lateral geniculate nucleus of a rat killed at 5 days after a unilateral visual cortex aspiration. Note dense degenerating axon terminals in the subgeniculate nucleus (SubG) (Section courtesy of Dr. E.-Y. Chen, Rush University [D]) (click to see enlarged photo).
Detection of neurodegeneration in the rat brain of a stroke model. 40-micron cryostat section was cut coronally through the striatum of a rat used as an animal model of stroke. The section was processed for demonstrating neuronal damage with NDT103 Kit. Note the accumulation of metallic silver grains (black) in both the striatum and the cortex, indicating the presence of neurodegeneration (click to see enlarged photo).
Detection of neurodegeneration in the rat brain of an epilepsy model. 40-micron cryostat section through the cortex of a rat used as an animal model of epilepsy. Note degenerating neurons (black) in the deep layers (click to see enlarged photo).