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NDT103
NDT101 NDT102 NDT104 NDT105

NDT103-1 FD NeuroSilverTM Kit
Price: $582.00 (For 300 sections)
NDT103-2 FD NeuroSilverTM Kit
Price: $437.25 (For 150 sections)

This Kit I is designed for the detection of degenerating neurons in fixed tissue sections of the central nervous system from experimental animals. The principle of this kit is based on the findings that some components of the neurons undergoing degeneration, such as lysosomes, axons, and terminals, become particularly argyrophilic. Under certain conditions, these cellular elements bind to the silver ions with high affinity. Upon reduction, the silver ions from metallic grains that are visible under a light or electron microscope.

NDT103 Kit has been used widely in animal studies under various experimental conditions1-16. This kit has proven extremely specific and sensitive for the detection of degenerating neuronal somata, axons, and terminals in both the brain and the spinal cord (see photo). It is particularly useful for the detection of small numbers of degenerating neurons that may not be demonstrable with routine histopathological techniques.

NDT103 Kit has also been proven to be very sensitive and reliable for the detection of amyloid plaques in the brain of transgenic mice (see photo). In addition, this kit may be used for demonstrating neurodegeneration and/or amyloid plaques in tissue sections that have been processed for immunohistochemistry (see photo & also ref. 2). The procedure of NDT103-1 Kit takes approximately 1 hour.

Contents:

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Solution A 500 ml
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Solution B 500 ml
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Solution C 500 ml
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Solution D 500 ml
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Solution E 2 ml
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Solution F 3 ml
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Solution G (5X) 500 ml
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Glass Specimen Retriever

Materials Required, but Not Included:

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Double distilled water
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Tissue culture plates (6-well).
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Histological supplies and equipment, including gelatin-coated microscope slides, glass coverslips, hair brush, staining jars, xylene or xylene substitutes and a light microscope.

References:

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Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov AV and Greenamyre JT: Chronic systemic pesticide exposure reproduces features of Parkinson's disease. Nature Neuroscience 3: 1301-1306, 2000.
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Sternfeld M, Shoham S, Klein O, Flores-Flores C, Evron T, Idelson GH, Kitsberg D, Patrick JW, and Soreq H: Excess 'read-through' acetylcholinesterase attenuates but the 'synaptic' variant intensifies neurodeterioration correlates. Proc. Natl. Acad. Sci. USA 97: 8647-8652, 2000.
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Kuhlmann AC and Guilarte TR: Cellular and subcellular localization of peripheral benzodiazepine receptors after trimethyltin neurotoxicity. J. Neurochem. 74: 1694-1704, 2000.
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Zito MA, Koennecke LA, McAuliffe MJ, McNally B, van Rooijen N and Heyes MP: Depletion of systemic macrophages by liposome-encapsulated clodronate attenuates striatal macrophage invasion and neurodegeneration following local endotoxin infusion in gerbils. Brain Res. 892:13-26, 2001.
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Northington FJ, Ferriero DM, Graham EM, Traystman RJ, and Martin LJ: Early neurodegeneration after hypoxia-ischemia in neonatal rat is necrosis while delayed neuronal death is apoptosis. Neurobiol. Dis. 8:207-219, 2001.
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Ding Y, Yao B, Lai Q and McAllister JP: Impaired motor learning and diffuse axonal damage in motor and visual systems of the rat following traumatic brain injury. Neurol. Res. 23: 193-202, 2001.
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Shoham S, Javitt DC and Heresco-Levy U: Chronic high-dose glycine nutrition: effects on rat brain cell morphology. Biol. Psychiatry 49: 876-885, 2001.
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Ding Y, McAllister JP, Yao B, Yan N and Canady AI: Axonal damage associated with enlargement of ventricles during hydrocephalus: a silver impregnation study. Neurol. Res. 23: 581-587, 2001.
11.
Northington FJ, Ferriero DM, and Martin LJ: Neurodegeneration in the thalamus following neonatal hypoxia-ischemia is programmed cell death. Dev. Neurosci. 23: 186-191, 2001.
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Ding Y, McAllister JP, Yao B, Yan N and Canady AI: Neuron tolerance during hydrocephalus. Neuroscience 106:659-667, 2001.
13.
Guo-Ross SX, Clark S, Montoya DAC, Jones KH, Obernier J, Shetty AK, White AM, Blusztajn JK, Wilson WA, and Swartzwelder HS: Prenatal choline supplementation protects against postnatal neurotoxicity. J. Neuroscience 22: RC195: 1-6, 2002.
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McCormack AL, Thiruchelvam M, Manning-Bog AB, Thiffault C, Langston JW, Cory-Slechta DA, and Di Monte DA: Environmental risk factors and Parkinson's disease: selective degeneration of nigral dopaminergic neurons caused by the herbicide paraquat. Neurobiol. Dis. 10: 119-127, 2002.
15.
Montoliu C, Humet M, Canales J_J, Burda J, Planells-Cases R, Sanchez-Baeza F, Carbonell T, Perez-Paya E, Messeguer A, Ferrer-Montiel A and Felipo V: Prevention of in vivo excitotoxicity by a family of trialkylglycines, a novel class of neuroprotectants. J. Pharmacol. Exp. Ther. 301: 29-36, 2002.
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Tong W, Igarashi T, Ferriero DM, and Noble LJ: Traumatic brain injury in the immature mouse brain: characterization of regional vulnerability. Exp. Neurol. 176: 105-116, 2002.
17.
Bianco CL, Ridet JL, Schneider BL, Deglon N, and Aebischer P: α--synucleinopathy and selective dopaminergic neuron loss in a rat lentiviral-based model of Parkinson's disease. Proc. Natl. Acad. Sci. USA 99: 10813-10818, 2002.
18.
Lauwers E, Debyser Z, Van Dorpe J, De Strooper B, Nuttin B, and BaekelaNDT V: Neuropathology and neurodegeneration in rodent brain induced by lentiviral vector-mediated overexpression of alpha-synuclein. Brain Pathol.13: 364-372, 2003.
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Shoham S, Bejar C, Kovalev E, and Weinstock M: Intracerebroventricular injection of streptozotocin causes neurotoxicity to myelin that contributes to spatial memory deficits in rats. Exp. Neurol. 184: 1043-1052, 2003.
20.
Manning-Bo? AB, McCormack AL, Purisai MG, Bolin LM, and Di Monte A: α-synuclein overexpression protects against paraguat-induced neurodegeneration. J. Neuroscience 23: 3095-3099, 2003.
21.
Paschen W, Yatsiv I, Shoham S, and Shohami E: Brain trauma induces X-box protein 1 processing indicative of activation of the endoplasmic reticulum unfolded protein response. J. Neurochem. 88: 983-992, 2004.

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